|本期目录/Table of Contents|

[1]周纪东,黄黄伟,朱城杰,等.一种测定原料肉中氨含量的分光光度法[J].温州职业技术学院学报,2016,02:60-63.
 ZHOU Jidong,HUANG Wei,ZHU Chengjie,et al.A Spectrophotometry to Test Ammonia Content in Meat Used as Raw Materials[J].Journal of Wenzhou Vocational and Technical College,2016,02:60-63.
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一种测定原料肉中氨含量的分光光度法(PDF)

《温州职业技术学院学报》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2016年02期
页码:
60-63
栏目:
应用技术
出版日期:
2016-05-01

文章信息/Info

Title:
A Spectrophotometry to Test Ammonia Content in Meat Used as Raw Materials
作者:
周纪东黄黄伟朱城杰魏晓玲
浙江育英职业技术学院生物技术系; 浙江两岸食品连锁有限公司;
Author(s):
ZHOU Jidong1 HUANG Wei2 ZHU Chengjie1 WEI Xiaoling2
1.Biotechnology Department, Zhejiang Yuying Vocational Technology College, Hangzhou, 310018, China; 2.Zhejiang Cross-straits Food Chain Co. Ltd., Hangzhou, 310018, China
关键词:
原料肉 新鲜度 分光光度法
Keywords:
Meat used as raw materials Ammonia Freshness Spectrophotometry
分类号:
O657.3;TS207.3
DOI:
10.13669/j.cnki.33-1276/z.2016.040
文献标识码:
A
摘要:
为判定原料肉的新鲜度,建立一种测定原料肉中氨含量的分光光度法。分光光度法以对羟基苯甲酸钠(PHB)为显色剂,研究原料肉中氨含量与吸光度的关系。结果表明,1.0m L肉浸液中加入3.0m L PHB溶液和1.5m L次氯酸钠溶液,于37℃水浴加热30min,在695nm处测定吸光度,在0.00~20.00μg/25m L范围内氨含量与吸光度呈现出良好的线性关系。分光光度法具有较高的准确度、精确度和灵敏度,操作简单,对仪器、试剂要求不高,可作为测定原料肉新鲜度的备选方法,面向中小型肉类加工企业推广与应用。
Abstract:
One new spectrophotometry is set up to test ammonia content in meat used as raw materials with the purpose of evaluating the freshness of the meat. By using P-hydroxybenzoic acid monosodium salt (PHB) as chro?mogenic agent, the relation between ammonia content and absorbance in the meat has been studied. Results show that to heat for 30minutes in water bath at 37 , the 1.0mL of meat extract bouillon added with 3.0mL of PHB solution and 1.5mL of sodium hypochlorite solution will turn out fine linear relation between ammonia content and absor?bance within 0.00 20.00 g/25mL when absorbance is measured 695 nm away. Characterized with its high precision and accuracy, sensitiveness, being easy to operate, and low demand for instruments and reagent, the spectropho-tometry can be used as an alternative for testing freshness of meat and can be introduced to small and medium meat processing enterprises and used there.

参考文献/References

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备注/Memo

备注/Memo:
【基金】 2015年度浙江省高等学校国内访问工程师校企合作项目(137);浙江省大学生科技创新活动计划(新苗人才计划)项目20(2016R453001);浙江育英职业技术学院院级课题(YYFW1501)
更新日期/Last Update: 2016-06-20