Integrating Zhejiang business culture into college students’ business quality education is important for colleges and universities to implement moral education and a necessary way to inherit Zhejiang spirit. Based on the “five dimensions” of Zhejiang business culture quality system, and deep study on the inheritance of Zhejiang business culture and entrepreneurship in colleges and universities in the new era, it is found that colleges and universities should give full play to the advantages of two-way communication between enterprises and colleges under the integration of education with industry, and integrate corporate culture, professional knowledge and professional cultural quality into the business quality education system and teaching plan. Meanwhile, it pays to educate students with literature, and explore and cultivate high-quality talents with professional knowledge and skills, humanistic emotion and business spirit in the new era.